IVF, ICSI, and IUI require the use of cleansed motile sperm cells. Semen samples contain numerous substances that are detrimental to eggs thereby the need to separate the motile sperm cells from the semen. Sperm cell preparation usually requires centrifugation (usually at least twice), washing, and swim-up to isolate the motile sperm cells.
The centrifugation steps in sperm washing are very stressful events for sperm cells. We use a technique that isolates motile sperm cells without any centrifugation at all to maintain sperm integrity and avoid damage caused by centrifugation.